biotinylated lectin (Vector Laboratories)

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Vector Laboratories biotinylated lectin

a <t>Lectin-cytokeratin</t> double staining at E16.5 showing normal (insert 1) and abnormal (insert 2) labyrinth morphology in Trpv2 −/− placentas. Scale bar = 400 μm and 50 μm. Representative images from n = 12–13 placentas per genotype from 8 independent litters. Similar results were observed in all Trpv2 −/− placentas analysed. b Immunohistochemistry of MCT1 and MCT4, representing differentiated SynTI and SynTII cells, respectively. Arrows indicate SynTI cells with altered morphology. Scale bar = 250 μm and 20 μm. Staining was performed on consecutive sections. Representative images from n = 5–6 placentas per genotype from 6 independent litters with similar results. c Enhanced volcano plot of differentially expressed genes identified by bulk RNA-sequencing of E14.5 labyrinth tissue ( Trpv2 +/+ versus Trpv2 −/− ; 111 downregulated and 56 upregulated genes). Differential expression analysis was performed using DESeq2 with Benjamini-Hochberg correction for multiple testing. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Marker genes for different labyrinth trophoblast populations are indicated. d qRT-PCR validation of selected differentially expressed genes identified by RNA-sequencing. Data are presented as mean values ± SEM. Statistical analysis was performed using two-sided Student’s t tests on ΔCt values. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Exact p-values are also provided in the file. e Cnetplot showing the association between differentially expressed genes and enriched Gene Ontology biological processes. f Overrepresentation analysis showing enrichment of SynTII marker genes among downregulated genes. Statistical significance was determined by hypergeometric testing with correction for multiple testing. g MERscope spatial transcriptomic overview of E16.5 placental sections from wild-type (WT) and Trpv2 -deficient placentas (KO1, KO2). Shown are Bdkrb2 (junctional zone), Pecam1 (fetal endothelial cells), and Vtcn1 (SynTII), illustrating overall placental architecture and organisation of major placental compartments used for subsequent region-based analyses. Scale bar = 1 mm h MERscope spatial transcriptomic overview illustrating region-based annotation of labyrinth tissue used for downstream analyses; wild-type (WT) labyrinth regions and two classes of knockout labyrinth regions: morphologically normal regions (NC) and abnormal regions lacking fetal capillaries (AbC). Scale bar = 1 mm i Heatmap of region-based pseudo-bulk expression profiles derived from MERscope analysis comparing WT, NC and AbC labyrinth regions. Samples and genes are ordered by unsupervised hierarchical clustering. Colour scale indicates relative expression (scaled z-score). j Representative MERscope expression maps of SynTI-associated markers ( Atp2a3, Tgfa and Syna ) and the SynTII precursor-associated marker Slc5a5 in WT and Trpv2 -deficient placentas at E16.5, illustrating the spatial distribution of trophoblast lineage markers in normal and abnormal labyrinth regions. Scale bars = 1 mm.

Biotinylated Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 669 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+gsl+i+isolectin+b4/Biotinylated+Griffonia+Simplicifolia+Lectin+I+(GSL+I)+isolectin+B4/pmc13654927-410-4-9
Average 95 stars, based on 669 article reviews

biotinylated lectin - by Bioz Stars, 2026-10

95/100 stars

Images

1) Product Images from "Placental TRPV2 supports trophoblast function and normal fetal development"

Article Title: Placental TRPV2 supports trophoblast function and normal fetal development

Journal: Nature Communications

doi: 10.1038/s41467-026-77576-z

a Lectin-cytokeratin double staining at E16.5 showing normal (insert 1) and abnormal (insert 2) labyrinth morphology in Trpv2 −/− placentas. Scale bar = 400 μm and 50 μm. Representative images from n = 12–13 placentas per genotype from 8 independent litters. Similar results were observed in all Trpv2 −/− placentas analysed. b Immunohistochemistry of MCT1 and MCT4, representing differentiated SynTI and SynTII cells, respectively. Arrows indicate SynTI cells with altered morphology. Scale bar = 250 μm and 20 μm. Staining was performed on consecutive sections. Representative images from n = 5–6 placentas per genotype from 6 independent litters with similar results. c Enhanced volcano plot of differentially expressed genes identified by bulk RNA-sequencing of E14.5 labyrinth tissue ( Trpv2 +/+ versus Trpv2 −/− ; 111 downregulated and 56 upregulated genes). Differential expression analysis was performed using DESeq2 with Benjamini-Hochberg correction for multiple testing. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Marker genes for different labyrinth trophoblast populations are indicated. d qRT-PCR validation of selected differentially expressed genes identified by RNA-sequencing. Data are presented as mean values ± SEM. Statistical analysis was performed using two-sided Student’s t tests on ΔCt values. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Exact p-values are also provided in the file. e Cnetplot showing the association between differentially expressed genes and enriched Gene Ontology biological processes. f Overrepresentation analysis showing enrichment of SynTII marker genes among downregulated genes. Statistical significance was determined by hypergeometric testing with correction for multiple testing. g MERscope spatial transcriptomic overview of E16.5 placental sections from wild-type (WT) and Trpv2 -deficient placentas (KO1, KO2). Shown are Bdkrb2 (junctional zone), Pecam1 (fetal endothelial cells), and Vtcn1 (SynTII), illustrating overall placental architecture and organisation of major placental compartments used for subsequent region-based analyses. Scale bar = 1 mm h MERscope spatial transcriptomic overview illustrating region-based annotation of labyrinth tissue used for downstream analyses; wild-type (WT) labyrinth regions and two classes of knockout labyrinth regions: morphologically normal regions (NC) and abnormal regions lacking fetal capillaries (AbC). Scale bar = 1 mm i Heatmap of region-based pseudo-bulk expression profiles derived from MERscope analysis comparing WT, NC and AbC labyrinth regions. Samples and genes are ordered by unsupervised hierarchical clustering. Colour scale indicates relative expression (scaled z-score). j Representative MERscope expression maps of SynTI-associated markers ( Atp2a3, Tgfa and Syna ) and the SynTII precursor-associated marker Slc5a5 in WT and Trpv2 -deficient placentas at E16.5, illustrating the spatial distribution of trophoblast lineage markers in normal and abnormal labyrinth regions. Scale bars = 1 mm.

Figure Legend Snippet: a Lectin-cytokeratin double staining at E16.5 showing normal (insert 1) and abnormal (insert 2) labyrinth morphology in Trpv2 −/− placentas. Scale bar = 400 μm and 50 μm. Representative images from n = 12–13 placentas per genotype from 8 independent litters. Similar results were observed in all Trpv2 −/− placentas analysed. b Immunohistochemistry of MCT1 and MCT4, representing differentiated SynTI and SynTII cells, respectively. Arrows indicate SynTI cells with altered morphology. Scale bar = 250 μm and 20 μm. Staining was performed on consecutive sections. Representative images from n = 5–6 placentas per genotype from 6 independent litters with similar results. c Enhanced volcano plot of differentially expressed genes identified by bulk RNA-sequencing of E14.5 labyrinth tissue ( Trpv2 +/+ versus Trpv2 −/− ; 111 downregulated and 56 upregulated genes). Differential expression analysis was performed using DESeq2 with Benjamini-Hochberg correction for multiple testing. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Marker genes for different labyrinth trophoblast populations are indicated. d qRT-PCR validation of selected differentially expressed genes identified by RNA-sequencing. Data are presented as mean values ± SEM. Statistical analysis was performed using two-sided Student’s t tests on ΔCt values. n = 6 placentas per genotype (3 male and 3 female) from 5 independent litters. Exact p-values are also provided in the file. e Cnetplot showing the association between differentially expressed genes and enriched Gene Ontology biological processes. f Overrepresentation analysis showing enrichment of SynTII marker genes among downregulated genes. Statistical significance was determined by hypergeometric testing with correction for multiple testing. g MERscope spatial transcriptomic overview of E16.5 placental sections from wild-type (WT) and Trpv2 -deficient placentas (KO1, KO2). Shown are Bdkrb2 (junctional zone), Pecam1 (fetal endothelial cells), and Vtcn1 (SynTII), illustrating overall placental architecture and organisation of major placental compartments used for subsequent region-based analyses. Scale bar = 1 mm h MERscope spatial transcriptomic overview illustrating region-based annotation of labyrinth tissue used for downstream analyses; wild-type (WT) labyrinth regions and two classes of knockout labyrinth regions: morphologically normal regions (NC) and abnormal regions lacking fetal capillaries (AbC). Scale bar = 1 mm i Heatmap of region-based pseudo-bulk expression profiles derived from MERscope analysis comparing WT, NC and AbC labyrinth regions. Samples and genes are ordered by unsupervised hierarchical clustering. Colour scale indicates relative expression (scaled z-score). j Representative MERscope expression maps of SynTI-associated markers ( Atp2a3, Tgfa and Syna ) and the SynTII precursor-associated marker Slc5a5 in WT and Trpv2 -deficient placentas at E16.5, illustrating the spatial distribution of trophoblast lineage markers in normal and abnormal labyrinth regions. Scale bars = 1 mm.

Techniques Used: Double Staining, Morphology, Immunohistochemistry, Staining, Bulk RNA Sequencing, Tissue, Quantitative Proteomics, Analysis, Marker, Quantitative RT-PCR, Biomarker Discovery, RNA Sequencing, Knock-Out, Expressing, Derivative Assay

a Mendelian inheritance pattern of litters obtained from Gcm1 -Cre wt/tg ; Trpv2 +/- crossed with female Trpv2 F/F mice. Data are shown as litter means ± SEM. b Fetal weight of Trpv2 SynTII-/- and Trpv2 SynTII+/+ fetuses at E16.5, E18.5 and birth. Box plots show the median (centre line); box boundaries indicate the 25th and 75th percentiles; whiskers indicate minimum and maximum values. Statistical analysis was performed using two-way ANOVA with Holm-Šídák’s multiple comparisons test. n = 11 litters at E16.5, n = 15 litters at E18.5 and n = 9 litters at term. c Placental weight of Trpv2 SynTII-/- and Trpv2 SynTII+/+ fetuses at E16.5 and E18.5. Box plots show the median (centre line); box boundaries indicate the 25th and 75th percentiles; whiskers indicate minimum and maximum values. Two-sided unpaired Student’s t test. n = 11 litters at E16.5 and n = 15 litters at E18.5. d Lectin-cytokeratin double staining of E18.5 Trpv2 SynTII-/- placentas showing regions lacking fetal capillaries. Scale bars, 500 μm and 50 μm. Representative images from n = 15 Trpv2 SynTII-/- placentas and n = 10 control placentas collected from 13 independent litters. Similar results were observed in all affected Trpv2 SynTII-/- placentas analysed. e Quantification of fetal capillary-void areas within the labyrinth at E18.5. Data were analysed using a two-sided unpaired Student’s t test. n = 10 control placentas and n = 15 Trpv2 SynTII-/- placentas from 13 independent litters. Exact p -values are also provided in the file.

Figure Legend Snippet: a Mendelian inheritance pattern of litters obtained from Gcm1 -Cre wt/tg ; Trpv2 +/- crossed with female Trpv2 F/F mice. Data are shown as litter means ± SEM. b Fetal weight of Trpv2 SynTII-/- and Trpv2 SynTII+/+ fetuses at E16.5, E18.5 and birth. Box plots show the median (centre line); box boundaries indicate the 25th and 75th percentiles; whiskers indicate minimum and maximum values. Statistical analysis was performed using two-way ANOVA with Holm-Šídák’s multiple comparisons test. n = 11 litters at E16.5, n = 15 litters at E18.5 and n = 9 litters at term. c Placental weight of Trpv2 SynTII-/- and Trpv2 SynTII+/+ fetuses at E16.5 and E18.5. Box plots show the median (centre line); box boundaries indicate the 25th and 75th percentiles; whiskers indicate minimum and maximum values. Two-sided unpaired Student’s t test. n = 11 litters at E16.5 and n = 15 litters at E18.5. d Lectin-cytokeratin double staining of E18.5 Trpv2 SynTII-/- placentas showing regions lacking fetal capillaries. Scale bars, 500 μm and 50 μm. Representative images from n = 15 Trpv2 SynTII-/- placentas and n = 10 control placentas collected from 13 independent litters. Similar results were observed in all affected Trpv2 SynTII-/- placentas analysed. e Quantification of fetal capillary-void areas within the labyrinth at E18.5. Data were analysed using a two-sided unpaired Student’s t test. n = 10 control placentas and n = 15 Trpv2 SynTII-/- placentas from 13 independent litters. Exact p -values are also provided in the file.

Techniques Used: Analysis, Double Staining, Control

Blocking Assay:

Article Title: Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells
Article Snippet: .. Samples were transferred to a blocking solution (2% BSA in PBS) at room temperature for 2 h. Then, tissues were blotted dry with gauze and incubated in PBS containing 0.5% Triton X-100 and a 1:75 dilution of Biotinylated GSL I—isolectin B4 (Vector Laboratories, Burlingame, CA), was applied at room temperature for 1 h with gentle agitation on a platform shaker. ..

Article Title: The TGF-β pathway is activated by 5-fluorouracil treatment in drug resistant colorectal carcinoma cells
Article Snippet: .. For the detection of mouse microvasculature, after deparaffinization, slides were blocked with Carbo-FreeTM Blocking Solution (Vector Laboratories) and incubated with Biotinylated GSL I –isolectin B4 (10 μg/ml, Vector Laboratories). ..

Incubation:

Article Title: Hyperhomocysteinemia disrupts retinal pigment epithelial structure and function with features of age-related macular degeneration
Article Snippet: .. Cells were then incubated for 3h at 37°C with anti-ZO1 (Life technology, Cat#: 40-2200402200, diluted at 1/200), anti-occludin (Invitrogen, Cat#: 331500, diluted at 1/200), anti-F-actin (Abcam, ab205, diluted at 1/200) or Biotinylated GSL I - isolectin B4 (Vector Laboratories, Burlingame, Ca), Cat#: B-1205, 7μl/ml). .. Thereafter, cells were washed 3 times with PBS containing 0.3% Triton-X, incubated with appropriate secondary antibodies (Alexafluor and Texas red avidin, Invitrogen, Eugene, Oregon), and coverslipped with Fluoroshield containing DAPI (Sigma-Aldrich Chemical Corp., St. Louis, MO) as a counter stain.

Gentle:

Immunohistochemistry:

Article Title: Cardiomyocyte p65 NF-κB Is Necessary for Compensatory Adaptation to Pressure-Overload
Article Snippet: .. Endothelial-specific immunohistochemistry staining was performed with Biotinylated GSL I–isolectin B4 (Vector Laboratories, Inc.) on freshly cut sections. ..

Article Title: Cardiomyocyte p65 NF-κB Is Necessary for Compensatory Adaptation to Pressure-Overload
Article Snippet: .. Microvascular density evaluation Endothelial-specific immunohistochemistry staining was performed with Biotinylated GSL I–isolectin B4 (Vector Laboratories, Inc.) on freshly cut sections. ..

Staining:

Article Title: Cardiac-Selective Expression of EcSOD After Systemic Injection of AAV9 Protects the Heart Against Post-MI LV Remodeling
Article Snippet: .. Capillaries were stained with biotinylated GSL I – isolectin B4 (5 μg/ml, Vector Laboratories). .. Neutrophils in heart sections were immunostained using rat monoclonal anti-mouse neutrophil antibody (Serotec, Raleigh, NC).

Affinity Purification:

Article Title: Characterization of immunogenic Neu5Gc in Bioprosthetic Heart Valves
Article Snippet: The commercial bioprostheses used in the study were kindly donated by the corresponding producing companies (Medtronic, Sorin, St. Jude Medical). .. Antibodies and Lectins We used affinity-purified polyclonal chicken anti-Neu5Gc IgY [ 40 ] (Biolegend), horse radish peroxidase (HRP)-streptavidin , Cy3-streptavidin, HRP-goat-anti-human IgG, Cy3-goat-anti-human IgG (H+L), biotinylated donkey-anti-chicken IgY, HRP-AffiniPure donkey-anti-chicken IgY (IgG)(H+L), Cy3-donkey-anti-chicken IgY (IgG)(H+L) (Jackson ImmunoResearch), biotinylated GSL I – isolectin B4 (IB4), biotinylated SNA ( Sambucus nigra ) and biotinylated MAL-II ( Maackia amurensis lectin II) (Vector Labs). ..

Article Title: Characterization of immunogenic Neu5Gc in Bioprosthetic Heart Valves
Article Snippet: The commercial bioprostheses used in the study were kindly donated by the corresponding producing companies (Medtronic, Sorin, St. Jude Medical). .. We used affinity-purified polyclonal chicken anti-Neu5Gc IgY [ 40 ] (Biolegend), horse radish peroxidase (HRP)-streptavidin , Cy3-streptavidin, HRP-goat-anti-human IgG, Cy3-goat-anti-human IgG (H+L), biotinylated donkey-anti-chicken IgY, HRP-AffiniPure donkey-anti-chicken IgY (IgG)(H+L), Cy3-donkey-anti-chicken IgY (IgG)(H+L) (Jackson ImmunoResearch), biotinylated GSL I – isolectin B4 (IB4), biotinylated SNA ( Sambucus nigra ) and biotinylated MAL-II ( Maackia amurensis lectin II) (Vector Labs). ..

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